讲座题目：Fluorescence Detection of Singlet Oxygen during Photodynamic Therapy（光动力学疗法中单线态氧的荧光检测）
主讲人：Tetsuro Majima日本大阪大学产业科学研究所教授
时间：2015-04-29 9:30
地点：校本部东区环化楼915会议室
报告人简介：Tetsuro Majima教授是国际著名的光化学家，日本大阪大学科学与工程研究所教授，兼任大阪大学材料分析中心主任。目前主要从事介孔光催化剂的制备及其在生物和环境方面的应用，单分子光化学反应、三维纳米复合材料制备与应用、DNA损伤与DNA动力学研究等。现任美国化学会著名刊物《Langmuir》高级编辑，在国际高水平杂志上发表各类学术论文百余篇，专著多部。
报告摘要：Singlet oxygen, molecular oxygen in the lowest excited state, has a critical role in the cell-killing mechanism of photohynamic therapy (PDT). Although singlet oxygen phosphorescence measurement has been mainly used to monitor singlet oxygen formation during PDT, its intensity is far insufficient to obtain two-dimensional images of intracellular singlet oxygen with the subcellular spatial resolution using the currently available near-IR detector. Here, we propose a new far-red fluorescence probe of singlet oxygen, namely, Si-DMA, composed of silicon-containing rhodamine and anthracene moieties as a chromophore and a singlet oxygen reactive site, respectvily. For the first time, we successfully visualized singlet oxygen generated during PDT with a spatial resolution of a single mitochondrial tubule.